Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors

TRYPAN BLUE

Product Specifications

Specifications Parameters
Dye Content (By Spectrophotometry) >70%
Transition Range Ph 4.3-6.3
Yellow-Pink
Ph 9.4-12.0
Brown Orange-Violet
Solubility 0.1% (Dist. Water) Clear Solution
Absorption Max (0.1N Naoh) Λ₁max 553-559Nm
Absorption Max (0.1N Naoh) Λ₂max 553-559Nm
Absorptivity (A1%/1Cm At 556 Λ₁max) >350
Absorptivity (A1%/1Cm At 595 Λ₂max) >325
Loss On Drying (110⁰C) <5%
Suitability For Microscopy Passes Test
Specifications Parameters
C.A.S. No. 72-57-1
C.I. No. 23850
Molecular Weight 960.82 g/mole
Solubility 0.1% (Water) Blue solution
Absorption maximum (in Methanol) 602 – 607nm
Absorptivity 1%/ 1cm, (in Methanol) > 625
Dye content (Spectrophotometry) > 60%
Loss On Drying at 110 degrees C (1hr) < 10%

Average Stock Availability (in Kg)

Trypan Blue

Trypan blue is a diazo dye used in ophthalmic cataract surgery to stain the anterior capsule in the presence of a mature cataract, to aid in visualization, before creating the continuous curvilinear capsulorhexis. Trypan Blue is commonly used as a stain to distinguish viable from non-viable cells. It turns dead cells blue and viable cells unstained. Trypan Blue is a known animal carcinogen and an experimental teratogen. A dye obtained from tolidine that is absorbed by the macrophages of the reticuloendothelial system and is therefore used for staining cells in biological research. Trypan blue is an antiprotozoal drug first used to treat Babesia infection in 1909 and still commonly used to treat Babesia canis. Trypan Blue is a water-soluble dye used for the dye exclusion test for cell viability to distinguish between viable and nonviable cells by seeping into nonviable cells with damaged plasma membranes causing them to appear blue. The method is sometimes referred to as the dye exclusion method. Trypan Blue Solution, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately. Trypan Blue is recommended for counting viable mammalian cells.
COA Download Form
Please Fill Out The Form For The Certificate Of Analysis

    faq's

    frequently asked questions

    We ship free-of-cost samples (freight free) for all our products. In fact, we recommend to approve a pre-shipment sample before purchase of product.
    The globally accepted specifications of Merck, Sigma-Aldrich and ACS are the benchmark for all our products. And we surpass the benchmark for all our products.
    We provide all the technical assistance in testing the product by providing detailed Method-of-Analysis, standard spec copy, any benchmark data if available. We also, assist with live testing of the product. We do not believe in room for ambiguity, hence, all assistance is provided till customer is satisfied with the end result.
    With a team of profound expertise, we have hands-on experience of closely working with Unilever, Milliken, DKSH, Biosynth, Sigma Aldrich (now Merck) and many more industry leaders to jointly develop some exceptional and one of the most innovative Healthcare products.
    Almost all of the products are available in stock in bulk quantities. In a rare case, we take a couple of weeks for preparation.
    We strive to provide the purest form of the compound. However, we welcome our customer’s in-house specs and try our best to suffice their in-house spec compliance.
    Yes, we gladly welcome the procurement and R&D team to visit our manufacturing facility for audit and even to run trials for product development and application. What are the various streams of products manufactured by you?
    We started with a couple of pH Indicators and Biological Stains, but over the last 40 years, we have expanded to Life Science products, pH Indicators, Complexometric Indicators, and even Specialty Colors which have a specialised performance driven application.
    We have a dedicated 1600 sqft research and development division for development of different molecules belonging to color changing chemistry which help in development of innovative end-use applications in different products.
    We are extremely sensitive towards the environment and polluted water generated from processes is self treated using MVR distillation process at our facility ensuring zero discharge.
    We generally submit quotes of freight. On approval, we dispatch goods and keep consignee updated with shipment status at every step. Alternatively, we also ship on customer’s courier account.
    We usually submit a Pre-shipment Sample to customer for quality approval. Although, most customer prefer a simple COA approval, but we insist on sample approval to avoid all post-commercial shipment hiccups.
    Though, its a rare case (Since all products are mostly ready in stock), we usually intimate buyer of the lead time for preparation of the product. Buyer is updated with the status from time to time. Once product is ready, a PSS is sent for quality approval. On approval, we dispatch the product.
    For every inquiry of the product received, we send the pre-shipment sample representing the lot for quality approval. Only after approval, the commercial shipments are dispatched. Any issues in the quality of the product are resolved at the sampling stage. For the test of the sample, all assistance is provided from our end, with sufficient data and documentation. This has helped us fireproof the quality-related issues and have faced negligible rejections so far.

    Submit Your Enquiry

    Scroll to Top