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Product Specifications

Specifications Parameters
Dye Content (By Spectrophotometry) >70%
Transition Range Ph 4.3-6.3
Ph 9.4-12.0
Brown Orange-Violet
Solubility 0.1% (Dist. Water) Clear Solution
Absorption Max (0.1N Naoh) Λ₁max 553-559Nm
Absorption Max (0.1N Naoh) Λ₂max 553-559Nm
Absorptivity (A1%/1Cm At 556 Λ₁max) >350
Absorptivity (A1%/1Cm At 595 Λ₂max) >325
Loss On Drying (110⁰C) <5%
Suitability For Microscopy Passes Test
Specifications Parameters
C.A.S. No. 2321-07-5
C.I. No. 45350:1
Molecular Weight 332.31g/mole
Solubility 0.1% (0.1N NaOH) Clear yellow solution with fluorescence
Absorption maximum(in 0.1N NaOH) 490 – 492nm
Absorptivity 1%/ 1cm,(in 0.1N NaOH) >2250
Loss On Drying at 110 degrees C (1hr) < 1%

Average Stock Availability (in Kg)


Fluorescein is also known as a color additive (D&C Yellow 7). Fluorescein is a diagnostic contrast agent particularly used in various ophthalmic procedures, such as checking for any corneal or vessel abnormalities. The application of fluorescein also extends to bioimaging whole anatomic structures and even further to cellular components in immunohistological staining. Fluorescein appears as yellow amorphous solid or orange-red crystals. Fluorescein is used forensically to detect traces of blood, in analytical chemistry as an indicator in silver nitrate titrations and in microscopy. It has a role as a radiopaque medium and a fluorescent dye. It is a xanthene dye, a gamma-lactone, a polyphenol, an oxaspiro compound, a member of 2-benzofurans and an organic hetero pentacyclic compound. It is functionally related to a fluorescein (acid form). Fluorescein, a phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium, such as the aqueous humor, and is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma. It has been approved by the FDA for use in externally applied drugs and cosmetics. Fluorescein is an organic compound that has wide use as a synthetic coloring agent. Fluorescein is an ophthalmological diagnostic agent used in an eye examination to detect the presence of lesions and foreign bodies in the eyes. The primary ingredient of Fluorescein is available as ophthalmic strips and injection. The ophthalmic strip is touched to the surface of the eye for staining the eye. The injectable form is given as a shot into a vein in the eye. This dye should be used only under the direct supervision of your ophthalmologist. Fluorescein angiography of the ocular fundus. This dye is used in an eye examination procedure to visualize the eye fundus region which helps to detect the presence of lesions and foreign bodies in the eyes.
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    frequently asked questions

    We ship free-of-cost samples (freight free) for all our products. In fact, we recommend to approve a pre-shipment sample before purchase of product.
    The globally accepted specifications of Merck, Sigma-Aldrich and ACS are the benchmark for all our products. And we surpass the benchmark for all our products.
    We provide all the technical assistance in testing the product by providing detailed Method-of-Analysis, standard spec copy, any benchmark data if available. We also, assist with live testing of the product. We do not believe in room for ambiguity, hence, all assistance is provided till customer is satisfied with the end result.
    With a team of profound expertise, we have hands-on experience of closely working with Unilever, Milliken, DKSH, Biosynth, Sigma Aldrich (now Merck) and many more industry leaders to jointly develop some exceptional and one of the most innovative Healthcare products.
    Almost all of the products are available in stock in bulk quantities. In a rare case, we take a couple of weeks for preparation.
    We strive to provide the purest form of the compound. However, we welcome our customer’s in-house specs and try our best to suffice their in-house spec compliance.
    Yes, we gladly welcome the procurement and R&D team to visit our manufacturing facility for audit and even to run trials for product development and application. What are the various streams of products manufactured by you?
    We started with a couple of pH Indicators and Biological Stains, but over the last 40 years, we have expanded to Life Science products, pH Indicators, Complexometric Indicators, and even Specialty Colors which have a specialised performance driven application.
    We have a dedicated 1600 sqft research and development division for development of different molecules belonging to color changing chemistry which help in development of innovative end-use applications in different products.
    We are extremely sensitive towards the environment and polluted water generated from processes is self treated using MVR distillation process at our facility ensuring zero discharge.
    We generally submit quotes of freight. On approval, we dispatch goods and keep consignee updated with shipment status at every step. Alternatively, we also ship on customer’s courier account.
    We usually submit a Pre-shipment Sample to customer for quality approval. Although, most customer prefer a simple COA approval, but we insist on sample approval to avoid all post-commercial shipment hiccups.
    Though, its a rare case (Since all products are mostly ready in stock), we usually intimate buyer of the lead time for preparation of the product. Buyer is updated with the status from time to time. Once product is ready, a PSS is sent for quality approval. On approval, we dispatch the product.
    For every inquiry of the product received, we send the pre-shipment sample representing the lot for quality approval. Only after approval, the commercial shipments are dispatched. Any issues in the quality of the product are resolved at the sampling stage. For the test of the sample, all assistance is provided from our end, with sufficient data and documentation. This has helped us fireproof the quality-related issues and have faced negligible rejections so far.

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