Product Specifications
| Specifications | Parameters |
|---|---|
| Dye Content (By Spectrophotometry) | >70% |
| Transition Range | Ph 4.3-6.3 |
| Yellow-Pink | |
| Ph 9.4-12.0 | |
| Brown Orange-Violet | |
| Solubility 0.1% (Dist. Water) | Clear Solution |
| Absorption Max (0.1N Naoh) Λ₁max | 553-559Nm |
| Absorption Max (0.1N Naoh) Λ₂max | 553-559Nm |
| Absorptivity (A1%/1Cm At 556 Λ₁max) | >350 |
| Absorptivity (A1%/1Cm At 595 Λ₂max) | >325 |
| Loss On Drying (110⁰C) | <5% |
| Suitability For Microscopy | Passes Test |
| Specifications | Parameters |
|---|
| C.A.S. No. | 314-13-6 |
| C.I. No. | 23860 |
| Molecular Weight | 960.82 g/mole |
| Solubility 0.1% (Dist. Water) | Clear blue solution |
| Absorption maximum (Dist. Water) | 605 – 611nm |
| Absorptivity 1%/ 1cm, (Dist. Water) | > 800 |
| Dye content (by Spectrophotometry) | > 85% |
| Loss On Drying at 110 degrees C (1hr) | < 10 % |
Average Stock Availability (in Kg)
- Upto 25
- 25 to 100
- 100 to 500
- Above 1000
Evan's Blue
Evans Blue is an azo dye which has a very high affinity for serum albumin. Due to its high water solubility and slow excretion, as well as its tight binding to serum albumin, Evans Blue has been widely used in biomedicine, including its use in estimating blood volume and vascular permeability, detecting lymph nodes, and localizing the tumor lesions. It can be useful in physiology in estimating the proportion of body water contained in blood plasma. Evans Blue Dye is widely used to study blood vessel and cellular membrane permeability as it is non-toxic, it can be administered as an intravital dye and it binds to serum albumin – using this as its transporter molecule.
The Evans Blue–albumin conjugate can be identified macroscopically by the striking blue color within tissue, observed by red auto-fluorescence in tissue sections examined by fluorescence microscopy and assessed and quantified by spectrophotometry for serum samples, or homogenized tissue. Evans Blue has recently been utilized in mdx mice to identify permeable skeletal myofibers that have become damaged as a result of muscular dystrophy.
Evans Blue has the potential to be a useful vital stain of myofibre permeability in other models of skeletal muscle injury and membrane-associated fragility. Evans Blue dye is used in microscopic or electron microscopic examination of cells and tissues to give contrast and to highlight particular features of interest, such as nuclei and cytoplasm.
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