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EVAN’S BLUE

Product Specifications

Specifications Parameters
Dye Content (By Spectrophotometry) >70%
Transition Range Ph 4.3-6.3
Yellow-Pink
Ph 9.4-12.0
Brown Orange-Violet
Solubility 0.1% (Dist. Water) Clear Solution
Absorption Max (0.1N Naoh) Λ₁max 553-559Nm
Absorption Max (0.1N Naoh) Λ₂max 553-559Nm
Absorptivity (A1%/1Cm At 556 Λ₁max) >350
Absorptivity (A1%/1Cm At 595 Λ₂max) >325
Loss On Drying (110⁰C) <5%
Suitability For Microscopy Passes Test
Specifications Parameters
C.A.S. No. 314-13-6
C.I. No. 23860
Molecular Weight 960.82 g/mole
Solubility 0.1% (Dist. Water) Clear blue solution
Absorption maximum (Dist. Water) 605 – 611nm
Absorptivity 1%/ 1cm, (Dist. Water) > 800
Dye content (by Spectrophotometry) > 85%
Loss On Drying at 110 degrees C (1hr) < 10 %

Average Stock Availability (in Kg)

Evan's Blue

Evans Blue is an azo dye which has a very high affinity for serum albumin. Due to its high water solubility and slow excretion, as well as its tight binding to serum albumin, Evans Blue has been widely used in biomedicine, including its use in estimating blood volume and vascular permeability, detecting lymph nodes, and localizing the tumor lesions. It can be useful in physiology in estimating the proportion of body water contained in blood plasma. Evans Blue Dye is widely used to study blood vessel and cellular membrane permeability as it is non-toxic, it can be administered as an intravital dye and it binds to serum albumin – using this as its transporter molecule. The Evans Blue–albumin conjugate can be identified macroscopically by the striking blue color within tissue, observed by red auto-fluorescence in tissue sections examined by fluorescence microscopy and assessed and quantified by spectrophotometry for serum samples, or homogenized tissue. Evans Blue has recently been utilized in mdx mice to identify permeable skeletal myofibers that have become damaged as a result of muscular dystrophy. Evans Blue has the potential to be a useful vital stain of myofibre permeability in other models of skeletal muscle injury and membrane-associated fragility. Evans Blue dye is used in microscopic or electron microscopic examination of cells and tissues to give contrast and to highlight particular features of interest, such as nuclei and cytoplasm.
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    faq's

    frequently asked questions

    We ship free-of-cost samples (freight free) for all our products. In fact, we recommend to approve a pre-shipment sample before purchase of product.
    The globally accepted specifications of Merck, Sigma-Aldrich and ACS are the benchmark for all our products. And we surpass the benchmark for all our products.
    We provide all the technical assistance in testing the product by providing detailed Method-of-Analysis, standard spec copy, any benchmark data if available. We also, assist with live testing of the product. We do not believe in room for ambiguity, hence, all assistance is provided till customer is satisfied with the end result.
    With a team of profound expertise, we have hands-on experience of closely working with Unilever, Milliken, DKSH, Biosynth, Sigma Aldrich (now Merck) and many more industry leaders to jointly develop some exceptional and one of the most innovative Healthcare products.
    Almost all of the products are available in stock in bulk quantities. In a rare case, we take a couple of weeks for preparation.
    We strive to provide the purest form of the compound. However, we welcome our customer’s in-house specs and try our best to suffice their in-house spec compliance.
    Yes, we gladly welcome the procurement and R&D team to visit our manufacturing facility for audit and even to run trials for product development and application. What are the various streams of products manufactured by you?
    We started with a couple of pH Indicators and Biological Stains, but over the last 40 years, we have expanded to Life Science products, pH Indicators, Complexometric Indicators, and even Specialty Colors which have a specialised performance driven application.
    We have a dedicated 1600 sqft research and development division for development of different molecules belonging to color changing chemistry which help in development of innovative end-use applications in different products.
    We are extremely sensitive towards the environment and polluted water generated from processes is self treated using MVR distillation process at our facility ensuring zero discharge.
    We generally submit quotes of freight. On approval, we dispatch goods and keep consignee updated with shipment status at every step. Alternatively, we also ship on customer’s courier account.
    We usually submit a Pre-shipment Sample to customer for quality approval. Although, most customer prefer a simple COA approval, but we insist on sample approval to avoid all post-commercial shipment hiccups.
    Though, its a rare case (Since all products are mostly ready in stock), we usually intimate buyer of the lead time for preparation of the product. Buyer is updated with the status from time to time. Once product is ready, a PSS is sent for quality approval. On approval, we dispatch the product.
    For every inquiry of the product received, we send the pre-shipment sample representing the lot for quality approval. Only after approval, the commercial shipments are dispatched. Any issues in the quality of the product are resolved at the sampling stage. For the test of the sample, all assistance is provided from our end, with sufficient data and documentation. This has helped us fireproof the quality-related issues and have faced negligible rejections so far.

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