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BIEBRICH SCARLET

Product Specifications

Specifications Parameters
Dye Content (By Spectrophotometry) >70%
Transition Range Ph 4.3-6.3
Yellow-Pink
Ph 9.4-12.0
Brown Orange-Violet
Solubility 0.1% (Dist. Water) Clear Solution
Absorption Max (0.1N Naoh) Λ₁max 553-559Nm
Absorption Max (0.1N Naoh) Λ₂max 553-559Nm
Absorptivity (A1%/1Cm At 556 Λ₁max) >350
Absorptivity (A1%/1Cm At 595 Λ₂max) >325
Loss On Drying (110⁰C) <5%
Suitability For Microscopy Passes Test
Specifications Parameters
C.A.S. No. 4196-99-0
C.I. No. 26905
Molecular Weight 556.49 g/mole
Solubility 0.1% (Dist. Water) Clear red solution
Absorption maximum (in Dist. Water) 506 – 510 nm
Absorptivity 1%/ 1cm (in Dist. Water) > 530
Dye content (by Spectrophotometry) > 70%
Loss On Drying at 110 degrees C (1hr) < 15%

Average Stock Availability (in Kg)

Biebrich Scarlet : Use & Applications

Biebrich scarlet is sometimes used as a plasma stain instead of Acid fuchsin in Masson’s trichrome. This dye should not be confused with the purified form of Sudan III, referred to as Biebrich scarlet R. Biebrich scarlet, is an organic sodium salt having 2-[(2-hydroxynaphthalen-1-yl)diazenyl]-5-[(4-sulfonatophenyl)diazenyl]benzene-1-sulfonate as the counterion. The stain, Biebrich scarlet, is photo fixing and is especially useful for identifying eosinophils by binding to their cytoplasmic granules in tissue samples from 6 to 60 microns in thickness. It also stains granules of macrophage and granular lymphocytes. Biebrich Scarlet, also known as Crocein scarlet, is also a molecule used in Lillie’s trichrome, a combination of dyes used in histology. Ungraded products supplied by Spectrum are indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or therapeutic use.
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    faq's

    frequently asked questions

    We ship free-of-cost samples (freight free) for all our products. In fact, we recommend to approve a pre-shipment sample before purchase of product.
    The globally accepted specifications of Merck, Sigma-Aldrich and ACS are the benchmark for all our products. And we surpass the benchmark for all our products.
    We provide all the technical assistance in testing the product by providing detailed Method-of-Analysis, standard spec copy, any benchmark data if available. We also, assist with live testing of the product. We do not believe in room for ambiguity, hence, all assistance is provided till customer is satisfied with the end result.
    With a team of profound expertise, we have hands-on experience of closely working with Unilever, Milliken, DKSH, Biosynth, Sigma Aldrich (now Merck) and many more industry leaders to jointly develop some exceptional and one of the most innovative Healthcare products.
    Almost all of the products are available in stock in bulk quantities. In a rare case, we take a couple of weeks for preparation.
    We strive to provide the purest form of the compound. However, we welcome our customer’s in-house specs and try our best to suffice their in-house spec compliance.
    Yes, we gladly welcome the procurement and R&D team to visit our manufacturing facility for audit and even to run trials for product development and application. What are the various streams of products manufactured by you?
    We started with a couple of pH Indicators and Biological Stains, but over the last 40 years, we have expanded to Life Science products, pH Indicators, Complexometric Indicators, and even Specialty Colors which have a specialised performance driven application.
    We have a dedicated 1600 sqft research and development division for development of different molecules belonging to color changing chemistry which help in development of innovative end-use applications in different products.
    We are extremely sensitive towards the environment and polluted water generated from processes is self treated using MVR distillation process at our facility ensuring zero discharge.
    We generally submit quotes of freight. On approval, we dispatch goods and keep consignee updated with shipment status at every step. Alternatively, we also ship on customer’s courier account.
    We usually submit a Pre-shipment Sample to customer for quality approval. Although, most customer prefer a simple COA approval, but we insist on sample approval to avoid all post-commercial shipment hiccups.
    Though, its a rare case (Since all products are mostly ready in stock), we usually intimate buyer of the lead time for preparation of the product. Buyer is updated with the status from time to time. Once product is ready, a PSS is sent for quality approval. On approval, we dispatch the product.
    For every inquiry of the product received, we send the pre-shipment sample representing the lot for quality approval. Only after approval, the commercial shipments are dispatched. Any issues in the quality of the product are resolved at the sampling stage. For the test of the sample, all assistance is provided from our end, with sufficient data and documentation. This has helped us fireproof the quality-related issues and have faced negligible rejections so far.

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