Product Specifications
| Specifications | Parameters |
|---|---|
| Dye Content (By Spectrophotometry) | >70% |
| Transition Range | Ph 4.3-6.3 |
| Yellow-Pink | |
| Ph 9.4-12.0 | |
| Brown Orange-Violet | |
| Solubility 0.1% (Dist. Water) | Clear Solution |
| Absorption Max (0.1N Naoh) Λ₁max | 553-559Nm |
| Absorption Max (0.1N Naoh) Λ₂max | 553-559Nm |
| Absorptivity (A1%/1Cm At 556 Λ₁max) | >350 |
| Absorptivity (A1%/1Cm At 595 Λ₂max) | >325 |
| Loss On Drying (110⁰C) | <5% |
| Suitability For Microscopy | Passes Test |
| Specifications | Parameters |
|---|
| C.A.S. No. | 7385-67-3 |
| Molecular Weight | 318.38 g/mole |
| Solubility 0.1% (Methanol) | Almost clear red solution with fluorescenes |
| Absorption maximum (Methanol) | 550 – 553nm |
| Absorptivity 1%/ 1cm, (Methanol) | >1200 |
| Loss On Drying at 110 degrees C (1hr) | < 3% |
Average Stock Availability (in Kg)
- Upto 25
- 25 to 100
- 100 to 500
- Above 1000
Nile Red
Nile Red is an uncharged hydrophobic molecule and a benzo phenoxazone dye. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments. Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in a hydrophobic environment, but shows minimal fluorescence in aqueous media.
Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red. Nile red can be used for the rapid determination of lipids by thin-layer chromatography or for staining whole tissue such as atherosclerotic blood vessels. Nile Red has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers.
It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red. Nile Red has been used for the staining of intracellular lipid droplets and neutral lipids.
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