ACID RED 33
C.I No. 17200
 Specifications:
Solubility in 0.1%(Water) Clear Solution
Absorptivity 1%/1cm at λ max 530.0nm < 650
Loss On Drying (110oC) > 5%
ALIZARINE COMPLEXONE
C.A.S. No. 3952 – 78 – 1
M. Wt. 412.36gm/mole
 Specifications:
Solubility 0.1% (0.1N NaOH) Clear solution
Absorption Maximum (0.1N NaOH) λ max. 564-568nm
Absorptivity (A1%, 1cm in Water, at λ max. > 250
Loss on drying (110oC) < 10%
ALIZARIN RED S
C.A.S. No. 130-22-3
CI NO. 58005
M.F. C14H6Na2O7S
M.W. 360.28gm/mole
 Specifications:
Dye content > 70%
Transition range pH4.3 – 6.3 Yellow – pink pH 9.4- 12.0 Brown orange- violet
Solubility 0.1%( water) Clear Solution
Absorption maximum in 0.1N NaOH λ1max 553-559 nm
Absorption maximum 0.1N NaOH λ2max 592- 599nm
Absorptivity (A1%/1cm at 556 λ1max) > 350
Absorptivity (A1%/1cm at 595 λ2max) > 325
Loss on drying (110oC) < 5 %
Suitability for microscopy To passes test
ALIZARIN YELLOW GG
CAS NO. 584-42-9
C.I. NO. 14025
M.F. C13H8N3O5Na
M.Wt. 309.22 gm/mole
 Specifications:
Dye content > 90%
Transition range pH 10.1 - 12.1 Brilliant Yellow - Brownish Yellow
Solubility in 0.1% (MeOH) Clear yellow solution
Absorption Max. (Methanol) λ max. 359-365nm
Absorptivity(A1%/1cm in Methanol, at λ max.) > 355
Loss on drying (110oC) < 5%
ALIZARIN YELLOW G (R)
CAS NO. 2243-76-7
C.I. NO. 14030
M.F. C13H9N3O5
M. Wt. 287.23gm/mole
 Specifications:
Dye content > 80%
Solubility in 0.1%(MeOH) Almost Clear Solution
Transition range pH 10.1 - 12.1 Yellow - Orange
Absorption Maximum(Methanol) λ max. 380 - 390 nm
Absorptivity (A1%/1cm in Methanol, at λ max) > 750
Loss on Drying (110oC) < 3%
ALUMINON (ACS)
CAS NO. 569-58-4
M.Wt. 473.44g/mole
M.F. (HOC6H3COONH4)2 C:C6H3(COONH4):O
 Specifications:
Solubility 0.1% (Water) Clear solution.
Insoluble matter < 0.1%
Residue after ignition < 0.2%
Sensitivity to aluminum To passes test
AMIDO BLACK 10 B
CAS NO. 1064 – 48 – 8
C. I. No. 20470
 Specifications:
Dye Content ( by Spectrophotometric) > 80%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist.Water) λ max. 617-620nm
Absorptivity (A1%, 1cm in Water, at λ max. > 750
Loss on drying (110oC) < 10%
Suitability for microscopy To passes test
ANILINE BLUE (W/S)
C.A.S. NO. 28983-56-4
C.I.NAME ACID BLUE 93
M.F. C37H27N3Na209S3
M.W. 799.80 gm/mole
 Specifications:
Solubility 0.1% (Dist. Water) Clear solution
Dye content (Spectrophotometric) > 50%
Absorption maximum (0.005M HCL) λmax 595-605nm
Absorptivity (A1%/1cm in 0.005M HCL, at λmax) > 550
Loss on drying (110oC) < 7%
AURIN (p-ROSOLIC ACID)
C.A.S. NO. 603 – 45 – 2
C.I. NO. 43800
M.F. C19H14O3
M.Wt. 290.32 gm/mole
 Specifications:
Dye content > 85%
Solubility 0.1% (Ethanol) Clear solution
Absorption maximum (Ethanol +5ml 1N HCl) λ max. 482 – 487 nm
Absorptivity (A1%/1cm, in Ethanol + 5ml 1N HCl at λ max.) > 2000
Loss on drying < 3%
AZUR A
C.A.S. NO. 531-53-3
C.I. NO. 52005
M.F. C14H14CIN3S
M.Wt. 291.80gm/mole
 Specifications:
Dye Content (Spectrophotometric) > 75%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist.Water) λ max. 628-633nm
Absorptivity (A1%, 1cm in Water, at λ max.On dried substance) > 1120
Loss on drying (110oC) < 8%
Suitability for microscopy To passes test
AZUR B (AZUR I)
C.A.S. NO. 531-55-5
C.I. NO. 52010
M.F. C15H16CIN3S
M.Wt. 305.83gm/mole
 Specifications:
Dye content 80%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum ( water) λ max. 645 – 650 nm
Absorptivity(A1%/1cm,in water at λ max. On dried substance) > 1600
Loss on drying (110oC) < 5%
Suitability for microscopy To passes test
AZUR C
C.A.S. NO. 531-57-7
M.F. C13H12Cl N3S
M.W. 277.78gm/mole
 Specifications:
Dye Content (Spectrophotometric) > 40%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist.Water) λ max. 613-619nm
Absorptivity (A1%, 1cm in Water, at λ max.On dried substance) > 1290
Loss on drying (110oC) < 8%
AZUR II
C.A.S. NO. 37247-10-2
 Specifications:
Solubility 0.1% (Dist. Water) Clear solution
Absorption maximum (Water) λ max 655 - 660nm
Absorptivity (A1%/1cm, in water, at λ max.On dried substance) > 1800
Loss on drying (110oC) < 12%
Suitability for microscopy To passes test
AZUR-II EOSINATE
C.A.S. NO. 53092-85-6
 Specifications:
Solubility 0.1% (MeOH) Clear solution
Absorption maximum (MeOH) λ1 max. 647-652nm
Absorption maximum (MeOH) λ2 max. 520-525nm
Absorptivity (A1%/1cm in MeOH,at λ1max.On dried substance) > 1250
Absorptivity (A1%/1cm in MeOH,at λ2 max. On dried substance) > 400
Loss on drying (110oC) < 10%
BIEBRICH SCARLET
C.A.S. NO. 4196 – 99 – 0
C.I. No. 26905
M.F. C22H14N4Na2O7S2
M. Wt. 556.49 gm/mole
 Specifications:
Dye Content (Spectrophotometric) > 70%
Solubility 0.1%(Water) Clear solution
Absorption maximum. (in water) λ max. 506 – 510 nm
Absorptivity (A1%, 1cm in water at λ max. on dried substance) 530 – 740
Loss on drying (110oC) < 15%
Suitability for microscopy To passes test
BRILLIANT CRESYL BLUE 65%
C.A.S. NO. 81029 – 05 – 2
M.F. C17H20CIN3O.1/2.ZnCl2
M. Wt. 385.96 gm/mole
 Specifications:
Dye Content (Spectrophotometric) > 65%
Solubility in 0.1% (50%Ethanol) Clear blue solution
Absorption Max. (in 50%Ethanol) λ max. 625- 630 nm
Absorptivity (A1%,1cm, in 50% Ethanol) > 1600
Loss on drying (110oC) < 8 %
Suitability for microscopy To passes test
BROMOCHLOROPHENOL BLUE SODIUM SALT
C.A.S. NO. 102185-52-4
M.F. C19H9Br2O5SNa
M. Wt. 603.06gm/mole
 Specifications:
Dye content > 90%
Solubility (in Waterl) Clear solution
Transition range pH 3.0–4.5
Greenish yellow – Blue - violet
Absorption maximum (pH 3.0) λ1 max 433 – 437 nm
Absorption maximum (pH 4.5) λ2 max 590 – 592 nm
Absorptivity (A1%/1cm at pH 3.0 at λ1 max) 370-400
Absorptivity (A1%/1cm at pH 4.5 at λ2 max) 900-1000
Loss on drying (110oC) < 5%
BROMOCRESOL GREEN (ACS)
C.A.S. NO. 76-60-8
M.F. C21H14Br4O5S
M. Wt. 698.04gm/mole
 Specifications:
Dye content > 95%
Transition range pH 3.8 – 5.4
Greenish yellow -Blue
Solubility 0.1%( 95%Ethanol) Clear solution
Absorption maximum (pH 3.8) λ1max 438 – 443 nm
Absorption maximum (pH 5.4) λ2max 615 – 618 nm
Absorptivity (A1%/1cm at pH 3.8 at λ1max) 240-270
Absorptivity (A1%/1cm at pH 5.4 at λ2max) 530-570
Loss on drying (110oC) < 3%
BROMOCRESOL GREEN SODIUM SALT (ACS)
C.A.S. NO. 62625-32-5
M.F. C21H13Br4NaO5S
M. Wt. 720.00gm/mole
 Specifications:
Dye content > 90%
Transition range pH 3.8 – 5.4
Greenish yellow - Blue
Solubility 0.1%( Water) Clear solution
Absorption maximum (pH 3.8) λ1max 438 – 443 nm
Absorption maximum (pH 5.4) λ2max 615 – 618 nm
Absorptivity (A1%/1cm at pH 3.8 at λ1max) 225-250
Absorptivity (A1%/1cm at pH 5.4 at λ2max) 500-540
Loss on drying (110oC) < 5%
BROMOCRESOL PURPLE (ACS)
C.A.S. NO. 115- 40 -2
M.F. C21H16Br2O5S
M. Wt. 540.24gm/mole
 Specifications:
Dye content > 95%
Transition range pH 5.2 – 6.8
Yellow – Purple
Solubility 0.1%( 95%Ethanol) Clear solution
Absorption maximum (pH 5.2) λ1max 427 – 431 nm
Absorption maximum (pH 6.8) λ2max 588 – 590 nm
Absorptivity (A1%/1cm at pH 5.2 at λ1max) 400 – 500
Absorptivity (A1%/1cm at pH 6.8 at λ2max) 1000 – 1100
Loss on drying (110oC) < 1%
BROMOCRESOL PURPLE SODIUM SALT (ACS)
C.A.S. NO. 62625-30-3
M.F. C21H15Br2SO5Na
M. Wt. 562.22gm/mole
 Specifications:
Dye content > 90%
Transition range pH 5.2 – 6.8
Yellow – Purple
Solubility 0.1%( Water ) Clear solution
Absorption maximum(in 0.1N, NaOH) λ max. 588-590 nm
Absorptivity (A1%/1cm, in 0.1N NaOH.) > 840
Loss on drying (110oC) < 5%
 
BROMOPHENOL BLUE (ACS)
C.A.S. NO. 115-39-9
M.F. C19H10Br4O5S
M. Wt. 669.99gm/mole
 Specifications:
Dye content > 95%
Solubility 0.1%(95% Ethanol) Clear solution
Transition range pH 3.0 – 4.6
Greenish yellow – Blue violet
Absorption maximum (pH 3.0) λ1max 434 – 439 nm
Absorption maximum (pH 4.6) λ2max 590 – 593 nm
Absorptivity (A1%/1cm at pH 3.8 at λ1max) 350 – 385
Absorptivity (A1%/1cm at pH 5.4 at λ2max) 940 - 1000
Loss on drying (110oC) < 1 %
BROMOPHENOL BLUE SODIUM SALT (ACS)
C.A.S. NO. 34725-61-6
M.F. C19H9Br4SO5Na
M. Wt. 691.97gm/mole
 Specifications:
Dye content > 90%
Solubility 0.1%( Water ) Clear solution
Transition range pH 3.0 – 4.6
Greenish yellow – Blue violet
Absorption maximum (pH 3.0) λ1max 434 – 439 nm
Absorption maximum (pH 4.6) λ2max 590 – 592 nm
Absorptivity (A1%/1cm at pH 3.0 at λ1max) 320 – 370
Absorptivity (A1%/1cm at pH 5.4 at λ2max) 880 - 980
Loss on drying (110oC) < 5 %
BROMOPHENOL RED
C.A.S. NO. 2800-80-8
M.F. C19H12Br2S
M. Wt. 512.19gm/mole
 Specifications:
Dye content > 95%
Solubility (in 95% Ethanol) Clear solution
Transition range pH 4.7 – 6.3
Yellow – Purple
Absorption maximum (pH 4.7) λ1max 435 – 440 nm
Absorption maximum (pH 6.3) λ2max 574 – 577 nm
Absorptivity (A1%/1cm at pH 4.7 at λ1max) 340 – 370
Absorptivity (A1%/1cm at pH 6.3 at λ2max) 585 – 720
Loss on drying (110oC) < 5 %
BROMOPYROGALLOL RED
C.A.S. NO. 16574-43-9
M.F. C19H10Br2O8S
M. Wt. 558.17gm/mole
 Specifications:
Solubility (in 95% Ethanol) Clear solution
Absorption maximum , λmax 554 – 557 nm
Absorptivity (A1%/1cm at pH 6.5 at λ1max) pH 4.7 – 6.3
Absorption maximum (pH 6.5) λ1max >750
Loss on drying (110oC) < 5 %
 
BROMOTHYMOL BLUE (ACS)
C.A.S. NO. 76-59-5
M.F. C27H28Br2O5S
M. W. 624.40gm/mole
 Specifications:
Dye content > 95%
Solubility 0.1%(95% Ethanol) Clear solution
Transition range pH 5.8 – 7.6 Yellow – Blue
Absorption maximum (pH 5.8) λ1max 430 – 435 nm
Absorption maximum (pH 7.6) λ2max 615 – 618 nm
Absorptivity (A1%/1cm at pH 5.8 at λ1max) 260 – 300
Absorptivity (A1%/1cm at pH 7.6 at λ2max) 470 – 520
Loss on drying (110oC) < 3 %
BROMOTHYMOL BLUE SODIUM SALT (ACS)
C.A.S. NO. 34722-90-2
M.F. C27H27Br2O5SNa
M. W. 646.38gm/mole
 Specifications:
Dye content > 90%
Solubility 0.1%( Water ) Almost clear solution
Transition range pH 5.8 – 7.6 Yellow – Blue
Absorption maximum (pH 5.8) λ1max 430 – 435 nm
Absorption maximum (pH 7.6) λ2max 615 – 618 nm
Absorptivity (A1%/1cm at pH 5.8 at λ1max) 250 – 280
Absorptivity (A1%/1cm at pH 7.6 at λ2max) 400 – 500
Loss on drying (110oC) < 5 %
BROMOXYLENOL BLUE
C.A.S. NO. 40070-59-5
M.F. C23H20Br2O5S
M. W. 568.29gm/mole
 Specifications:
Solubility (in 95% Ethanol) Clear solution
Transition range pH 5.7 – 7.4 Yellow – Blue
Absorption maximum (pH 5.7) λ1max 432 – 437 nm
Absorption maximum (pH 7.4) λ2max 613 – 615 nm
Absorptivity (A1%/1cm at pH 5.7 at λ1max) 300 – 330
Absorptivity (A1%/1cm at pH 7.4 at λ2max) : 500 – 540
Loss on drying (110oC) < 3 %
Dye content > 95%
CALCEIN (FLUORESCEIN COMPLEXONE)
C.A.S. NO. 1461 – 15 – 0
M.F. C30H22N2O13
M. W. 622.55gm/mole
 Specifications:
Solubility 0.1%(0.1N NaOH) Clear solution
Absorption maximum (0.1 NaOH) λ1max 499 +/-5nm
Absorptivity 1%/ 1cm, at λ max. > 800
Loss on drying at 110oC (1hr.) < 8 %
CALCON (SOLOCHROME DARK BLUE)
C.A.S. NO. 2538-85-4
C.I. No. 15705
Chemical Formula C20H13N2NaO5S.
Molecular Wt. 416.39gm/mole
 Specifications:
Solubility 0.1% (50% ethanol) Clear solution
Absorption maximum. (Ammonia buffer pH 12.2 + 2ml EDTA solution) λ max 632-636nm
Absorptivity (A1%,1cm pH 12.2,at λ max on dried substance) > 300
LOD (110oC) < 10 %
Solubility (in dist. Water) Passes test
CALCON CARBOXYLIC ACID (PATTON & READER’S REAGENT)
C.A.S. NO. 3737-95-9
Chemical Formula C21H14N2O7S.
Molecular Wt. 438.41gm/mole
 Specifications:
Solubility 0.1% (MeOH) Clear solution
Absorption maximum (Methanol+1ml 1N HCL) λmax 550-560nm
Absorptivity (A1%1cm in Methanol+ 1ml 1N HCL, at λmax) > 250
Loss on drying (105oC) < 7%
Suitability as indicator for Metal Titration To passes test
Dye Content ( by Spectrophotometric) > 60%
CALMAGITE
C.A.S. NO. 3147-14-6
Chemical Formula C17H14N2O5S
Molecular Wt. 358.37gm/mole
 Specifications:
Solubility 0.1% (Dist. Water) Clear solution
Absorption maximum (pH 10.0 in Ammonia buffer + 2ml of 0.05 M E.D.T.A. Solution) λmax 600-610nm
Absorptivity (A1% 1cm at λmax, at pH 10.0 in Ammonia buffer +2ml of 0.05M E.D.T.A. solution on dried substance) > 300
Loss on drying (110oC) < 10%
CARBOL FUCHSIN
C.A.S. NO. 4197-24-4
 Specifications:
Solubility 0.1 % (50% Ethanol) Clear solution
Absorption maximum (50% Ethanol) λmax 547-552nm
Absorptivity (A1%1cm in 50%Ethanol, at λmax) >2150
Loss on drying (110oC) <10%
CHROMOTROPIC ACID DISODIUM SALT (ACS)
C.A.S. NO. 5808-22-0
Chemical Formula  C10H6N2O8S2 .2H2O
Molecular Wt.   400.29gm/mole
 Specifications:
Clarity of solution 5% ( in water + 0.5ml 10% NaOH) To Passes test
Sensitivity to nitrate To Passes test
Sensitivity to formaldehyde To Passes test
CHLOROPHENOL RED 93%
C.A.S. NO. 4430-20-0
Chemical Formula  C19H12Cl2O5S.
Molecular Wt.   423.32gm/mole
 Specifications:
Solubility (in 95% Ethanol) Clear solution
Transition Range pH 4.8 – 7.0
Absorption maximum (0.1N NaOH) 573 – 579 nm
Absorptivity (A1%1cm ,in 0.1NaOH) >1550
Loss on drying (110oC) < 7%
Dye Content ( by Spectrophotometric) > 93%
CHROMOTROPE 2B
C.A.S. NO. 548-80-1
M.F.  C16H9N3Na2O10S2
C.I.NO.  16575
M.W. 513.37gm/mole
 Specifications:
Dye content >75%
Solubility 0.1 % (Water ) Clear red solution
Absorption maximum (Water) λmax 512-518nm
Absorptivity (A1%1cm in Water, at λmax) > 575
Loss on drying (110oC) < 8%
CHROMOTROPE 2R
C.A.S. NO. 4197 – 07 – 3
Chemical Formula  C16H10N2Na2O8S2
C.I.NO.  16570
M.W. 468.37 gm/mole
 Specifications:
Dye content >75%
Solubility 0.1 % (in methanol ) Clear red solution
Absorption maximum (Methanol) λ max 510 nm ± 4 nm
Absorptivity (A1%1cm in Methanol) > 485
Loss on drying (110oC) < 10%
CONGO RED
C.A.S. NO. 573 – 58 – 0
C.I.NO.  22120
 Specifications:
Transition range pH 3.0 – 5.2 Violet – orange red
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist.Water) λ max. 495-499nm
Absorptivity (A1%, 1cm in Water, at λ max > 520
Loss on drying (110oC) < 5%
Dye Content ( by Spectrophotometric) >75%
Suitability for microscopy To passes test
o-CRESOLPHTHALEIN (PURE)
C.A.S. NO. 596-27-0
M.F.  C22H18O4
M.W. 346.38 gm/mole
 Specifications:
Dye content > 95%
Appearance White – off white powder
Solubility 0.1% (EtOH) Clear solution
Transition range pH 8.2-9.8 Colorless – Red
Absorption maximum (0.1N NaOH) at λmax 564-568nm
Absorptivity (A1%1cm in 0.1N NaOH at λmax) > 1670
Loss on drying (110oC) < 1%
o-CRESOLPHTHALEIN COMPLEXON
C.A.S. NO. 2411-89-4
M.F.  C32H32N2O12
M.W. 636.62 gm/mole
 Specifications:
Solubility 0.1% (0.1N NaOH) Clear solution
Absorption maximum (0.1N NaOH) at λmax 572-578nm
Absorptivity (A1%1cm in 0.1N NaOH, at λmax On dried substance) >790
Loss on drying (110oC) < 6%
CRESOL RED (ACS)
C.A.S. NO. 1733-12-6
M.F.  C21H18O5S
M.Wt. 382.43gm/mole
 Specifications:
Dye content  > 95%
Solubility 0.1% (95% EtOH) Clear solution
Transition range pH 6.5-8.8 Yellow – Purple
Absorption maximum (pH 6.5) λ3max 432 – 436 nm
Absorption maximum (pH 8.8) λ4max 571 – 574 nm
Absorptivity (A1%1cm at pH 6.5 at λ3max) 490 – 540
Absorptivity (A1%1cm at pH 8.8 at λ4max) 1000 – 1100
Loss on drying (110oC)   < 3 %
CRESOL RED SODIUM SALT (ACS)
C.A.S. NO. 62625-29-0
M.F.  C21H17O5SNa
M.Wt. 404.42gm/mole
 Specifications:
Dye content  > 90%
Solubility 0.1% (Dist. Water) Clear solution
Transition range pH 6.8-8.8 Yellowish – Purple
Absorption maximum (pH 6.8) λ3max 432 – 437 nm
Absorption maximum (pH 8.8) λ4max 571 – 575 nm
Absorptivity (A1%1cm at pH 6.8 at λ3max) 460 – 520
Absorptivity (A1%1cm at pH 8.8 at λ4max) 950 – 1050
Loss on drying (110oC)   < 5 %
m – CRESOL PURPLE
C.A.S. NO. 2303-01-7
M.F.  C21H18O5S
M.Wt. 382.43gm/mole
 Specifications:
Appearance  Green powder
Transition range pH 7.4 – 9.0 Brownish yellow – Violet
Solubility 0.1%(50% EtOH) Clear Solution
Absorptivity 1%,1cm, ( 0.1 N NaOH, at λ max. ) 900- 1000
Loss On Drying at 110oC(1hr.) < 1%
CROCEIN ORANGE G
C.A.S. NO. 1934-20-9
C.I.NO. 15970
Chemical Formula C16H11N2SO4Na
M.Wt. 350.33gm/mole
 Specifications:
Dye content  >70 %
Solubility in 0.1% (in Dist. Water) Clear orange Solution
Absorpion maximum (in water) 482-486nm
Absorptivity 1%/1cm, at λ max > 460
Loss on drying (110oC) < 5%
4’5’- DIBROMOFLUORESCEIN
C.A.S. NO. 596-03-2
C.I.NO. 45370.1
M.F. C20H10BrO5
M.W. 332.31gm/mole
 Specifications:
Dye content  >90 %
Solubility 0.1 % (Methanol+1ml 1N HCL ) Clear Solution
Absorption Maximum(Methanol+1ml 1N HCL) λmax 445-450 nm
Absorptivity (A1% 1cm in, Methanol+1ml 1N HCL at λmax) > 300
Loss on drying (110oC) < 3%
 
2,6-DICHLOROPHENOL INDOPHENOL SODIUM SALT (ACS)
C.A.S. NO. 620 – 45 – 1
Chemical Formula C12H6Cl2NNaO22H2O
M.Wt. 326.11 gm/mole
 Specifications:
Solubility 0.1% ((50% EtOH +2ml Na2CO3 Clear solution
Absorption maximum (50% EtOH +2ml Na2CO3) λ max 640 – 645 nm
Absorptivity (A1%/1cm, in 50% EtOH +2ml Na2CO3), at λ max > 700
Interfering dyes ( acc.to ACS) To passes test
Loss on drying (120oC) < 12%
EOSIN BLUE
C.A.S. NO. 548-24-3
C.I. NO. 45400
M.F. C20H6Br2N2Na2O9
M.W. 624.09gm/mole
 Specifications:
Dye content (Gravimetric)  > 85%
Solubility 0.1 % (Water ) Clear solution
Absorption maximum (water) λmax 516-520nm
Absorptivity (A1%1cm in water at λmax) > 800
Loss on drying (110oC) < 8%
Suitability for microscopy To passes test
EOSIN YELLOW ( W/S)
C.A.S. NO. 17372-87-1
C.I. NO. 45380
M.F. C20H6Br4Na2O5
M.Wt. 691.86gm/mole
 Specifications:
Dye content (Spectrophotometry)  > 90%
Solubility 0.1 % (Water ) Clear solution
Absorption Maximum (water) λmax 515 - 518 nm
Absorptivity (A1%1cm in water at λmax) > 1260
Loss on drying (110oC) < 8%
Suitability for microscopy To passes test
ERIOCHROME BLACK T (ACS)(SOLOCHROME BLACK T)
C.A.S. NO. 1787-61-7
C.I. NO. 14645
M.F. C20H12N3NaO7S
M.Wt. 461.38gm/mole
 Specifications:
Solubility 0.1 % (Water ) Clear solution
Absorption Maximum (at pH 10.0 Ammonia Buffer + 2ml 0.05M E.D.T.A.) λmax 610-630 nm
Absorptivity (A1%,1cm at λmax at pH10.0 Ammonia Buffer + 0.05M E.D.T.A.) > 300
Loss on drying (110oC) < 1%
Suitability as metal indicator To passes test
ETHYL ORANGE
C.A.S. NO. 62758-12-7
M.F. C16H18N3SO3Na
M.Wt. 355.39gm/mole
 Specifications:
Dye Content > 95%
Solubility 0.1 % (Water) Clear solution
Transition range pH 3.0 - 4.8 Red – Orange Yellow
Absorption maximum (water) λmax 472- 477nm
Absorptivity (A1% 1cm in water, at λmax) > 850
Loss on drying (110oC) < 5%
EVAN’S BLUE
C.A.S. NO. 314-13-6
C.I. NO. 23860
M.F. C34H24N6Na4O14S4
M.W. 960.82gm/mole
 Specifications:
Dye Content >85%
Solubility 0.1 % (Water ) Clear solution
Absorption maximum (water) λmax 605-611nm
Absorptivity (A1% 1cm in water, at λmax On dried substance) > 800
Loss on drying (110oC) < 10%
Suitability for microscopy To passes test
GIEMSA'S STAIN
C.A.S. NO. 51811-82-6
 Specifications:
Solubility 0.1 % ( MeOH ) Clear solution
Absorption Maximum(in MeOH) λ1max 640 - 650 nm
Absorption Maximum(in MeOH) λ2max 520 - 525 nm
Absorptivity (A1%1cm in MeOH at λ1max On dried substance ) 950 -1150
Absorptivity (A1%1cm in MeOH at λ2max On dried substance ) 600 -750
Loss on drying (100oC) < 10 %
Suitability for microscopy To passes test
HYDROXY NAPHTHOL BLUE (ACS)
C.A.S. NO. 165660-27-5
M.F. C20H12N2O11Na2S3
M.W. 598.50 gm/mole
 Specifications:
Solubility 0.1 % (Water ) Clear solution
Suitability for calcium determination To passes test
Absorption maximum (Ammonia buffer pH 12.0 + 2ml 0.05M E.D.T.A.) 645-650nm
Absorptivity (A1% 1cm at λmax, in ammonia Buffer pH 12.0 + 2ml 0.05M E.D.T.A.) > 350
Loss on drying (110oC) < 5%
JENNER'S STAIN
C.A.S. NO. 62851-42-7
 Specifications:
Solubility 0.1 % (MeOH ) Clear solution
Absorption maximum (Methanol) λ1max 650-653nm
Absorption maximum (Methanol) λ2max 520-525nm
Absotptivity (A1%/1cm in Methanol, at λ1max On dried substance) >1100
Absotptivity (A1%/1cm in Methanol, at λ2max On dried substance) >600
Loss on drying (110oC) <10%
Suitability for microscopy To passes test
LEISHMAN'S STAIN
C.A.S. NO. 12627-53-1
 Specifications:
Solubility 0.1 % (MeOH ) Clear solution
Absorption Maximum (MeOH) λ1max 640-645 nm
Absorption Maximum (MeOH) λ2max 522-525nm
Absorptivity (A1%/1cm in MeOH, at λ1max On dried substance) > 900
Absorptivity (A1%/1cm in MeOH, at λ2max On dried substance) > 650
Loss on drying (110oC) <8%
Suitability for microscopy To passes test
MAGCOL CG
 Specifications:
Transition range Colorless - Green
pH 6.5 – 10.5
Solubility 0.1% (EtOH) Clear solution
Absorption Maximum (pH 10.5) λ1max 399-403 nm
Absorption Maximum (pH 10.5) λ2max 592-596 nm
Absorptivity 1%, 1cm,pH 10.5 at λ1 > 850
Absorptivity 1%, 1cm,pH 10.5 at λ2 > 350
Loss on drying at 110oC (1hr) < 5%
MAGCOL CP
 Specifications:
Transition range Colorless – Purple
pH 8.0 – 11.0
Solubility 0.1% (EtOH) Clear solution
Absorption Maximum (0.1N NaOH) λmax 567-573 nm
Absorptivity 1%, 1cm,(0.1NaOH) > 1200
Loss on drying at 110oC (1hr) < 3%
MAGCOL CY
 Specifications:
Transition range Colorless – Yellow
pH 5.0 – 7.0
Solubility 0.1% (EtOH) Clear solution
Absorption Maximum (0.1N NaOH) λmax 399-403 nm
Absorptivity 1%, 1cm,(0.1NaOH) > 550
Loss on drying at 110oC (1hr) < 3%
MAGCOL OG
 Specifications:
Transition range Orange – Green
pH 4.0 – 7.5
Solubility 0.1% (EtOH) Clear solution
Absorption Maximum (0.1N NaOH) λmax 1 614-618 nm
Absorption Maximum (0.1N NaOH) λmax 2 406-410 nm
Absorptivity 1%, 1cm, (0.1NaOH) λ1 > 375
Absorptivity 1%, 1cm, (0.1NaOH) λ2 > 350
Loss on drying at 110oC (1hr) < 3%
MAGCOL RYB
 Specifications:
Transition range Red – Yellow
pH 3.5 – 6.5
Yellow - Blue
pH 6.5 -9.0
Solubility 0.1% (EtOH) Clear solution
Absorption Maximum (in pH 3.5) λmax 1 518--523 nm
Absorption Maximum ((in pH 6.5) λmax 2 428- 434 nm
Absorption Maximum ((in pH 9.0) λmax 3 615- 618 nm
Absorptivity 1%, 1cm,( in pH 3.5) λ1 > 450
Absorptivity 1%, 1cm,( in pH 6.5) λ2 > 300
Absorptivity 1%, 1cm,( in pH 9.0) λ3 >550
Loss on drying at 110oC (1hr) < 3%
 
MAGCOL WRG 2
 Specifications:
Transition range Wine red – Green
pH 3.5 – 7.5
Solubility 0.1% (EtOH) Clear solution
Absorption Maximum (in pH 3.5) λmax 1 614-618 nm
Absorption Maximum ((in pH 7.5) λmax 2 406-410 nm
Absorptivity 1%, 1cm,( in pH 3.5) λ1 > 600
Absorptivity 1%, 1cm,( in pH 7.5) λ2 > 350
Loss on drying at 110oC (1hr) < 5%
MAGCOL YV 6
 Specifications:
Transition range Yellow – Violet
pH 5.0 – 8.0
Solubility 0.1% (95% EtOH) Clear solution
Absorption Maximum (in pH 5.0) λmax 1 430-434 nm
Absorption Maximum ((in pH 8.0) λmax 2 588-595 nm
Absorptivity 1%, 1cm,( in pH 5.0) λ1 > 350
Absorptivity 1%, 1cm,( in pH 8.0) λ2 > 900
Loss on drying at 110oC (1hr) < 3%
MAGCOL YV 7
 Specifications:
Transition range Yellow – Violet
pH8.0 – 10.5
Solubility 0.1% (95% EtOH) Clear solution
Absorption Maximum (in pH 8.0) λmax 1 430-434 nm
Absorption Maximum ((in pH 10.5) λmax 2 551-554 nm
Absorptivity 1%, 1cm,( in pH 8.0) λ1 > 100
Absorptivity 1%, 1cm,( in pH 10.5) λ2 > 900
Loss on drying at 110oC (1hr) < 3%
MAGCOL YV B
 Specifications:
Transition range Yellow – Violet blue
pH 5.0 – 8.0
Solubility 0.1% (95% EtOH) Clear solution
Absorption Maximum (in pH 5.0) λmax 1 428-434 nm
Absorption Maximum ((in pH 8.0) λmax 2 588-592 nm
Absorptivity 1%, 1cm,( in pH 8.0) λ1 > 400
Absorptivity 1%, 1cm,( in pH 10.5) λ2 >1000
Loss on drying at 110oC (1hr) < 3%
MAGCOL YGB
 Specifications:
Solubility 0.1%(Water) Almost clear solution
Transition range Yellow – Blue
pH 5.8 – 7.6
Absorption Maximum (in pH 5.0) λmax 1 430 – 435 nm
Absorption maximum (pH 7.6) λ2max 615 – 618 nm
Absorptivity (A1%/1cm at pH 5.8 at λ1max) > 250
Absorptivity (A1%/1cm at pH 7.6 at λ2max) > 400
Loss on drying (110oC) < 5 %
MAGCOL YB
 Specifications:
Transition range Yellow – Blue
pH 4.5 – 7.8
Solubility 0.1% (EtOH) Clear solution
Absorption Maximum (0.1N NaOH) λmax 1 588-594 nm
Absorption Maximum (0.1N NaOH) λmax 2 378-382 nm
Absorptivity 1%, 1cm,(0.1NaOH) λ1 > 900
Absorptivity 1%, 1cm,(0.1NaOH) λ2 > 100
Loss on drying at 110oC (1hr) < 3%
MAGNESON I
C.A.S. NO. 74 – 39 – 5
M. Wt. 259.22gm/mole
 Specifications:
Solubility 0.05%(Methano l+ 1ml 0.1N NaOH) Clear solution
Absorption Max. %(Methanol + 1ml 0.1N NaOH 442 +/- 3nm
Absorptivity (A1%, 1cm, at λ max.) > 1430
Loss on drying (110oC) < 8%
Dye Content ( by Spectrophotometric) > 90%
MAY & GRUNWALD STAIN
C.A.S. NO. 62851-42-7
 Specifications:
Solubility 0.1 % (MeOH ) Clear solution
Absorption maximum (Methanol) λ1max 650-653nm
Absorption maximum (Methanol) λ2max 520-525nm
Absotptivity (A1%/1cm in Methanol, at λ1max On dried substance) > 1100
Absotptivity (A1%/1cm in Methanol, at λ2max On dried substance) > 600
Loss on drying (110oC) < 10%
Suitability for microscopy To passes test
METHYL ORANGE (ACS)
C.A.S. NO. 547-58-0
C.I. NO. 13025
M.F. C14H14N3SO3Na
M.W. 327.34gm/mole
 Specifications:
Dye content > 95%
Solubility 0.1 % (Water ) Clear solution
Transition range pH 3.1- 4.4 Pink-Orange-Yellow
Abxorption maximum (pH 3.1) λ1max 501-504nm
Abxorption maximum (pH 4.4) λ2max 467-471nm
Absorptivity maximum (A1%/1cm at pH 3.1, at λ1max) 1050-1150
Absorptivity maximum (A1%/1cm at pH 4.4, at λ2max) 750-850
Loss on drying (110oC) < 3%
PHENOLPHTHALEIN COMPLEXON
C.A.S. NO. 25296-54-2
M.F. C30H28N2O12
M.W. 608.56gm/mole
 Specifications:
Appearance Off white powder
Solubility 0.1% (in 0.1N NaOH) Clear solution
Absorption Maximum(in 0.1N NaOH) λmax 556-560nm
Absorptivity (A1%/1cm, in 0.1N NaOH at λmax) >300
Absorptivity maximum (A1%/1cm at pH 3.1, at λ1max) 467-471nm
Loss on Drying (110oC) <5%
PHENOL RED (ACS)
C.A.S. NO. 143-74-8
M.F. C19H14O5S
M.W. 354.38gm/mole
 Specifications:
Dye Content > 95%
Solubility 0.1 % (95% EtOH) Clear solution
Transition Range pH 6.5 - 8.2
Yellow - Reddish Violet
Absorption Maximum (pH 6.5) λ1max 430 - 435nm
Absorption maximum (pH 8.2) λ2max 557 - 560nm
Absorptivity (A1%/1cm at pH 6.5 at λ1max) 600 - 700
Absorptivity (A1%/1cm at pH 8.2 at λ2max) 1100 - 1200
Loss On Drying (110oC) < 1%
PHENOL RED SODIUM SALT (ACS)
C.A.S. NO. 34487-61-1
M.F. C19H13O5SNa
M.W. 376.36 gm/mole
 Specifications:
Dye Content > 90%
Solubility 0.1 % (Water) Clear solution
Transition Range pH 6.5 - 8.2
Yellow - Reddish Violet
Absorption Maximum (pH 6.5) λ1max 430 - 435nm
Absorption maximum (pH 8.2) λ2max 557 - 560nm
Absorptivity (A1%/1cm at pH 6.5 at λ1max) 560 - 620
Absorptivity (A1%/1cm at pH 8.2 at λ2max) 970 - 1100
Loss On Drying (110oC) < 5%
PHLOXINE B
C.A.S. NO. 18472-87-2
C.I. NO. 45410
M.F. C20H2O5Cl4Br4Na2
M.W. 829.66 gm/mole
 Specifications:
Dye content (Gravimetric) > 80%
Solubility 0.1 % (Water + 2 ml1% Na2CO3 ) Clear solution
Absorption max. (water + 2 ml 1% Na2CO3 ) λmax 536-542nm
Absorptivity (A1%/1cm in, Water 2 ml1% Na2CO3) > 1100
Loss on drying (110oC) < 10%
Suitability for microscopy To passes test
PONCEAU S
C.A.S. NO. 6226-79-5
C.I. NO. 27195
M.F. C22H12N4Na4O13S4
M.W. 760.58gm/mole
 Specifications:
Dye content (Spectrophotometric) >75%
Solubility 0.1 % (Water) Clear solution
Absorption maximum (water) λmax 517-523 nm
Absorptivity (A1%/1cm in water, at λmax) 460-600
Loss On Drying (1100C) 10%
Suitability for microscopy To passes test
PYROGALLOL RED
C.A.S. NO. 32638-88-3
M.F. C19H14O9S
M.W. 418.38gm/mole
 Specifications:
Solubility 0.1% (0.1N NaOH) Clear solution
Absorption maximum ( in acidic methanol) λmax 474-478 nm
Absorptivity 1%, 1cm,in acidic methanol, at λ max. > 450
Loss on drying at 110oC (1hr) < 5%
PYROCATECHOL VIOLET
C.A.S. NO. 115-41-3
M.F. C19H14O7S
M.W. 386.38gm/mole
 Specifications:
Solubility 0.1% (Methanol) Clear solution
Absorption maximum (MeOH) λmax 442-446 nm
AAbsorptivity 1%, 1cm, at λ max. > 360
Loss on drying at 110oC (1hr) < 7%
ROSE BENGAL
C.A.S. NO. 632-69-9
C.I. NO. 45440
M.F. C20H2O5Cl4I4Na2
M.W. 1017.65 gm/mole
 Specifications:
Dye content (Spectrophotometry) > 90%
Solubility 0.1 % (Water) Clear solution
Absorption maximum (water + 2ml 5%Na2CO3Soln.) λmax 544-550
Absorptivity (A1%/1cm in water, at λmax) > 910
Loss on drying (110oC) < 10%
SODIUM DIPHENYLAMINE SULFONATE (ACS)
C.A.S. NO. 6152-67-6
M.F. C12H10NNaO3S
M.W. 271.27 gm/mole
 Specifications:
Solubility 0.1 % (Water) Clear solution %
Sulfated ash 26.5-28.5%
Absorption maximum (water) λmax 290-295nm
Absorptivity (A1%/1cm in water, at λmax on dried substance) 700-800
Sensitivity as indicator Passes test
Loss on drying (110oC) < 5%
SPADNS
C.A.S. NO. 23647-14-5
M.F. C16H9N2Na3O11S3
M.W. 570.42gm/mole
 Specifications:
Dye content > 80%
Solubility 0.1 % (Water) Clear solution
Absorption Maximum (water, at pH 7.0)λmax 505-510nm
Absorptivity (A1%/1cm in water, at pH 7.0 at λmax) > 375
Loss on drying (110oC) < 10%
SUDAN III
C.A.S. NO. 85-86-9
C.I. NO. 26100
M.F. C22H16N4O
M.W. 352.40gm/mole
 Specifications:
Dye conent (Spectrophotometric) >85%
Solubility 0.1 % (Toluene) Clear solution
Absorption maximum (Toluene) λmax 508-512nm
Absorptivity (A1%/1cm in Toluene, at λmax) > 870
Loss on drying (110oC) <1%
SUDAN IV
C.A.S. NO. 85-83-6
C.I. NO. 26105
M.F. C24H20N4O
M.W. 380.45gm/mole
 Specifications:
Dye content (Spectrophotometric) >80%
Solubility 0.1 % (Toluene) Clear solution
Absorption maximum (Toluene) λmax 520-523nm
Absorptivity (A1%/1cm in Toluene, at λmax) > 710
Loss on drying (110oC) < 1%
THYMOL BLUE (ACS)
C.A.S. NO. 76-61-9
M.F. C27H30O5S
M.W. 466.60 gm/mole
 Specifications:
Dye Content > 95%
Solubility 0.1 % (95% EtOH) Clear solution
Transition Range pH 7.8 – 9.5
Yellow - Blue
Absorption maximum (pH 7.8) λ1max 431-436nm
Absorption maximum (pH 9.5) λ2max 594-598nm
Absorptivity (A1%/1cm at pH 7.8, at λ1max) 200-300
Absorptivity (A1%/1cm at pH 9.5, at λ2max) 450-550
Loss on drying (110oC) < 3%
THYMOL BLUE SODIUM SALT (ACS)
C.A.S. NO. 62625-21-2
M.F. C27H29SO5Na
M.W. 488.58 gm/mole
 Specifications:
Dye Content > 90%
Solubility 0.1 % (Water) Clear solution
Transition Range pH 7.8 – 9.5
Yellow - Blue
Absorption maximum (pH 7.8) λ1max 431-437nm
Absorption maximum (pH 9.5) λ2max 594-598nm
Absorptivity (A1%/1cm at pH 7.8, at λ1max) 190-285
Absorptivity (A1%/1cm at pH 9.5, at λ2max) 425-525
Loss on drying (110oC) < 5%
THYMOLPHTHALEIN (ACS)
C.A.S. NO. 125-20-2
M.F. C28H30O4
M.W. 430.54 gm/mole
 Specifications:
Transition range pH 9.3-10.5
Colourless – Blue
Solubility 0.1% (95% Ethanol) Clear solution
Absorption maximum (0.1N NaOH) λmax 592-596nm
Absorptivity (A1%/1cm at 0.1NNaOH, at λmax) > 1000
Loss on drying (110oC) < 1%
THYMOLPHTHALEIN COMPLEXON
C.A.S. NO. 1913-93-5
M.F. C38H44O12N2
M.W. 720.78 gm/mole
 Specifications:
Solubility 0.1% (95% Ethanol) Clear solution
Absorption maximum (0.1N NaOH) λmax 600-605nm
Absorptivity (A1%/1cm 0.1N NaOH, at λmax On dried substance) > 650
Loss on dr